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Distribution and function of endothelial cells and relative content of collagen fibers, elastic fibers and smooth muscle in ligamentum teres hepatis. A: The ligamentum teres hepatis (LTH) lumen (× 100 magnification); B: Endothelial cells (black arrow, × 400 magnification); C: LTH after Verhoeff-Van Gieson (VVG) staining (× 400 magnification); D: The portal vein (PV) after VVG staining. Elastic fibers (red), collagen fibers (black) and smooth muscle (yellow) (× 400 magnification); E: CD34 expression in LTH endothelial cells (× 400 magnification); F: CD34 expression in PV endothelial cells (× 400 magnification); G: Factor VIII-related antigen (FVIIIAg) expression in LTH endothelial cells (× 400 magnification); H: FVIIIAg expression in PV endothelial cells (× 400 magnification); I: Endothelial nitric oxide synthase (eNOS) expression in LTH endothelial cells (× 400 magnification); J: eNOS expression in PV endothelial cells (× 400 magnification); K: Tissue type plasminogen activator (t-PA) expression in LTH endothelial cells (× 400 magnification); L: Tissue type plasminogen activator expression in PV endothelial cells (× 400 magnification).

Journal: World Journal of Gastrointestinal Surgery

Article Title: Ligamentum teres hepatis as a graft for portal and/or superior mesenteric vein reconstruction: From bench to bedside

doi: 10.4240/wjgs.v15.i4.674

Figure Lengend Snippet: Distribution and function of endothelial cells and relative content of collagen fibers, elastic fibers and smooth muscle in ligamentum teres hepatis. A: The ligamentum teres hepatis (LTH) lumen (× 100 magnification); B: Endothelial cells (black arrow, × 400 magnification); C: LTH after Verhoeff-Van Gieson (VVG) staining (× 400 magnification); D: The portal vein (PV) after VVG staining. Elastic fibers (red), collagen fibers (black) and smooth muscle (yellow) (× 400 magnification); E: CD34 expression in LTH endothelial cells (× 400 magnification); F: CD34 expression in PV endothelial cells (× 400 magnification); G: Factor VIII-related antigen (FVIIIAg) expression in LTH endothelial cells (× 400 magnification); H: FVIIIAg expression in PV endothelial cells (× 400 magnification); I: Endothelial nitric oxide synthase (eNOS) expression in LTH endothelial cells (× 400 magnification); J: eNOS expression in PV endothelial cells (× 400 magnification); K: Tissue type plasminogen activator (t-PA) expression in LTH endothelial cells (× 400 magnification); L: Tissue type plasminogen activator expression in PV endothelial cells (× 400 magnification).

Article Snippet: Mouse monoclonal anti-human CD34, rabbit monoclonal anti-human factor VIII-related antigen (FVIIIAg), rabbit polyclonal anti-human endothelial nitric oxide synthase (eNOS), and rabbit polyclonal anti-human tissue type plasminogen activator (t-PA) antibodies were purchased from Santa Cruz Biotechnology (CA, United States).

Techniques: Staining, Expressing

A multimode optical fiber was coated with a gold layer, a self-assembling monolayer (SAM) and the appropriate antigen (PAI-1), as shown in the inset. The SPR shift caused by the nanoparticles, coated with MA-33H1F7 or MA-T12D11, is clearly visible.

Journal: PLoS ONE

Article Title: Heterobifunctional PEG Ligands for Bioconjugation Reactions on Iron Oxide Nanoparticles

doi: 10.1371/journal.pone.0109475

Figure Lengend Snippet: A multimode optical fiber was coated with a gold layer, a self-assembling monolayer (SAM) and the appropriate antigen (PAI-1), as shown in the inset. The SPR shift caused by the nanoparticles, coated with MA-33H1F7 or MA-T12D11, is clearly visible.

Article Snippet: The monoclonal antibodies (host: mouse) used in this study are MA-33H1F7 (target: human PAI-1/t-PA complex) and MA-T12D11 (target: human TAFI), supplied by the Therapeutic and Diagnostic Antibodies group of the KU Leuven. . All ultrasonication steps were performed in a Branson 5510 sonicator bath.

Techniques: